Characters involved : Desdemona, Emilia, Clown, Othello, Iago, Cassio, Bianca.
The scene opened when Desdemona asked the clown where her husband was and only then she realized that she had lost her favourite handkerchief. When Othello approaches her, he asked her for her missing handkerchief and she did not manage to give him an answer. When Othello left, Iago and Cassio came in; Iago then asked her if Othello was mad at her and he too left. Emilia and Desdemona discussed about Othello's personality precisely regarding his jealousy. Then, Bianca comes in to meet Cassio, he gave her the handkerchief of Desdemona which he found in his chamber, and insisted her to make a copy of it as the design is nice. The scene ended when he became angry at Bianca as she assumed that he got the handkerchief from some other woman. However, Bianca was not disturbed and left with it.
"Lend me thy handkerchief"
"Here my lord"
"That which I gave you"
"I have it not about me" -p127
Source : The Arden Edition of the Works of William Shakespeare